{"id":731,"date":"2021-03-28T20:15:47","date_gmt":"2021-03-28T18:15:47","guid":{"rendered":"https:\/\/h5p.it.ntnu.no\/hbio3005\/?page_id=731"},"modified":"2021-05-16T15:57:06","modified_gmt":"2021-05-16T13:57:06","slug":"pasm","status":"publish","type":"page","link":"https:\/\/h5p.it.ntnu.no\/hbio3005\/pasm\/","title":{"rendered":"PASM"},"content":{"rendered":"\n

PASM (Periodic Acid Silver Methenamine) er en spesialfargemetode som benyttes for \u00e5 p\u00e5vise karbohydrater. Metoden benyttes blant annet for \u00e5 synliggj\u00f8re basalmembraner i glomerulikapill\u00e6rene i nyrevev (1).<\/p>\n\n\n\n

Oppvarmet periodsyre benyttes for \u00e5 oksidere karbohydrater til aldehyder. S\u00f8lvl\u00f8sningen benytter aldehydene som festepunkt. Aldehydene reduserer s\u00f8lvionene til metallisk s\u00f8lv. Videre utf\u00f8res toning ved \u00e5 tilsette gullklorid som reduserer s\u00f8lvmengden i preparatet ved \u00e5 oksidere s\u00f8lvet, se reaksjonslikning. Dette gir vevet permanent svart farge.<\/p>\n\n\n\n

3Ag(s) <\/sub>+ Au3+ <\/sup>\u2192 3Ag+<\/sup> + Au(s)<\/sub>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 <\/p>\n\n\n\n

Natriumthiosulfat tilsettes for \u00e5 fjerne rester\/overskudd av s\u00f8lv- og gullioner. Vevet farges til slutt med hematoxylin og erytrosin (1).<\/p>\n\n\n\n

Ved \u00e5 benytte PASM vil basalmembraner farges brun\/svart, kjerner farges bl\u00e5 og andre strukturer farges lilla (2).<\/p>\n\n\n\n

Nyresnitt farget med PASM, 400x forst\u00f8rrelse<\/strong><\/p>\n\n\n\n

<\/div>\n\n\n\n

L\u00e6r mer om hvordan ulike nyresnitt farget med PASM ser ut her<\/a><\/em>.<\/p>\n\n\n\n

Referanser<\/strong><\/p>\n\n\n\n

1:<\/strong> Bancroft, John D., Gamble, Marilyn. Theory and Practice of Histological Techniques. 6. utgave. Elsevier, Churchill Livingstone; 2008.<\/p>\n\n\n\n

2:<\/strong> Kiernan, J. A. Histological and Histochemical Methods, Theory and Practice. 4. utgave. Bloxham, Oxfordshire: Scion Publishing Ltd; 2008. <\/p>\n","protected":false},"excerpt":{"rendered":"

PASM (Periodic Acid Silver Methenamine) er en spesialfargemetode som benyttes for \u00e5 p\u00e5vise karbohydrater. Metoden benyttes blant annet for \u00e5 synliggj\u00f8re basalmembraner i glomerulikapill\u00e6rene i nyrevev (1). Oppvarmet periodsyre benyttes for \u00e5 oksidere karbohydrater til aldehyder. S\u00f8lvl\u00f8sningen benytter aldehydene som festepunkt. Aldehydene reduserer s\u00f8lvionene til metallisk s\u00f8lv. Videre utf\u00f8res toning ved \u00e5 tilsette gullklorid som … <\/p>\n

Fortsett \u00e5 lese \u00abPASM\u00bb<\/span><\/a><\/p>\n","protected":false},"author":53,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-731","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/h5p.it.ntnu.no\/hbio3005\/wp-json\/wp\/v2\/pages\/731","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/h5p.it.ntnu.no\/hbio3005\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/h5p.it.ntnu.no\/hbio3005\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/h5p.it.ntnu.no\/hbio3005\/wp-json\/wp\/v2\/users\/53"}],"replies":[{"embeddable":true,"href":"https:\/\/h5p.it.ntnu.no\/hbio3005\/wp-json\/wp\/v2\/comments?post=731"}],"version-history":[{"count":12,"href":"https:\/\/h5p.it.ntnu.no\/hbio3005\/wp-json\/wp\/v2\/pages\/731\/revisions"}],"predecessor-version":[{"id":2016,"href":"https:\/\/h5p.it.ntnu.no\/hbio3005\/wp-json\/wp\/v2\/pages\/731\/revisions\/2016"}],"wp:attachment":[{"href":"https:\/\/h5p.it.ntnu.no\/hbio3005\/wp-json\/wp\/v2\/media?parent=731"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}